『别名』Acetyl Coenzyme A Carboxylase alpha (phospho S80); p-Acetyl Coenzyme A Carboxylase alpha (phospho S80); ACAC; ACACA; ACACA; ACACA_HUMAN; ACC alpha; ACC; ACC-alpha; ACC1; ACC1; ACCA; acetyl CoA carboxylase 1; acetyl Coenzyme A; Acetyl Coenzyme A; Biotin carboxylase; Acetyl-Coenzyme A Carboxylase alpha.
『浓度』1mg/1ml
『规格』0.1ml/100μg
『抗体来源』Rabbit
『克隆类型』polyclonal
『交叉反应』Human, Mouse, Rat
『产品类型』一抗 磷酸化抗体
『研究领域』 心血管 细胞生物 转录调节因子
『蛋白分子量』predicted molecular weight: 265kDa
『性状』Lyophilized or Liquid
『免疫原』KLH conjugated synthesised phosphopeptide derived from human Acetyl Coenzyme A Carboxylase alpha around the phosphorylation site of Ser80
『亚型』IgG
『纯化方法』affinity purified by Protein A
『储存液』Preservative: 15mM Sodium Azide, 磷酸化乙酰辅酶A羧化酶抗体特价 Constituents: 1% BSA, 0.01M PBS, pH 7.4
『产品应用』WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
『保存条件』Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
『产品介绍』Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Function : Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
Subunit : Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.
Subcellular Location : Cytoplasm.
Tissue Specificity : Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
Post-translational modifications : Phosphorylation on Ser-1263 is required for interaction with BRCA1.
DISEASE : Defects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:613933]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.
Similarity : Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. Contains 1 carboxyltransferase domain.
Database links : UniProtKB/Swiss-Prot: Q13085.2
A Kit ，规格： 96T/48T